PROM TIES PL aE EME Coa” EM SR ES cate Ee Ne ON YD te: Lae neers 
Pie ae 
1909] LIPMAN—EFFECTS OF SALTS ON BACILLUS 119 
typical instance may be cited experiment 1 of their series, in which 
about 13°° of an m/1 solution of MgCl, was injected subcutaneously. 
Less than half an hour later there was produced general anaesthesia, 
with all the attending symptoms. When 2°° of a solution of m/8 
CaCl, was injected into the ear vein the rabbit was again breathing 
normally, and when 8°¢ had been given the animal sat up and appeared 
entirely recovered, except for a stiffness in the hind legs. 
In these experiments, some of which were even more striking than 
the one cited, Mettzer and Aver employed, besides the chlorids of 
Ca and Mg, the acetate and nitrate of the former, and the acetate, 
nitrate, and sulfate of the latter. The same strong antagonism was 
noted in all cases. 
TABLE VIII 
ALL QUANTITIES GIVEN REFER TO CUBIC CENTIMETERS OF 0.35 ™ SOLUTIONS 
Culture solution pages gm Miligais 
Too Mp le hia eas K 4.76 
ig — { ie eaieaes a J 4.48 
ties es BPE pte A I 4.20 
x00 MaCla i 3.78 
pt eae Ga G 3.64 
foo MgCl Fo r ce 
So Mel, p 5.08 
oa nie: eae D 3-29 
Bo MaCh c 343 
ote B 3-37 
100 CaGhy i ets te A 3-78 
In addition to the confirmation of the results stated above in 
experiments with the same material, one series was also carried out 
with a culture of B. subtilis obtained from New Jersey, and with 
salt solutions made up from a different grade of chemically, pure salt. 
