124 BOTANICAL GAZETTE [avoust 
the fourth the least. This is quite different from the results with 
higher plants, where magnesium is the most toxic and calcium the 
least. 
2. A marked antagonism exists between Ca and K, Mg and Nag 1 
K and Na. . 
3. No antagonism exists between Mg and Ca, but the toxic effect 
of each is increased by addition of the other to it. This is just the E 
opposite of what has hitherto been found for plants. 
In conclusion, the writer desires to express his indebtedness to — 
Prof. W. J. V. OsTeRHOUT, at whose instance these researches were — 
begun, for helpful suggestions and kindly criticism throughout the 5 
course of the investigations. 4 
_ LABORATORY OF SorL BACTERIOLOGY 
UNIVERSITY OF CALIFORNIA 
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