4 
172 BOTANICAL GAZETTE [SEPTEMBER 
reestablish the uninucleate condition. Multinucleate jacket cells 
are reported by Lawson (23) in various Cupressineae, also by CoutL- 
TER and LAND (11) in Torreya, and by Kirpaut (18) in Phyllocladus. 
Apparently no case has previously been reported, however, in whic 
a multinucleate prothallus persists in the conifers, but the record 
may easily have been overlooked by the writer, owing to a large pro- 
portion of the literature not being available to him. LAwson (23); 
however, makes no mention of such a case. Parallel cases are 
reported among the Gnetales (LAND 19, PEARSON 28), but this in 
itself is probably of little importance, especially as the origin of such 
cells is different in these cases. | 
The development of the embryo shows no peculiarities and closely 
resembles the general sequence of events as described for other con! 
fers, an apical cell being organized for a very short time (fg. 18); 
the presence of embryonal tubes is to be noted in jig. 19. In the 
mature embryo the cotyledons are two (very rarely three) in number 
and usually of the same length; sometimes, however, one cotyledon 
is conspicuously shorter than the other. 
COMPARISON WITH WIDDRINGTONIA WHYTEI AND CALLITRIS 
One collection of W. Whytei, growing in the Tokai plantations, 
was made on January 11, 1909, but did not yield results of much 
importance. It clearly agrees with W. cupressoides in the presenc® 
of laterally placed archegonia and in the multinucleate endosperm: 
Single collections were also made of three species of Callitris 
(C. verrucosa, C. cupressiforme, and C. M uelleri)+ during the second 
week in January. Of four ovules of the first-named species ae 
one showed secondary prothalli (text fig. 1), and in each the proth 
were in the free nuclear condition and one pollen tube only was foun a 
which had penetrated a short way down the side of the prothall = 
and contained three nearly equal nuclei (text fig. 3). The ¥PP 
of the three, however, stains more sharply than the other two - 
suggests the same sequence of events as described above for Widdring 
0s 
tonia. ‘The ovules of the other two species contained young mae 
in different stages of development (similar to figs. 17 -19). any- 
unfertilized archegonia are much too disorganized to make out 
4 See footnote, ee yf 2 
peers 
TAP ee Pea ee 
SE ee eI 
