332 BOTANICAL GAZETTE [NOVEMBER 
the more convex side, where it gave the zoospore the appearance of 
being a “hunch back.”” Such zoospores would perform very curious 
evolutions in their vain efforts to throw off the offending appendix, 
but in no case observed did this separation take place after the zoo- 
spores had separated from the group at the mouth of the exit tube. 
The number of zoospores from a single zoosporangium is 2-8, as 
stated above, or possibly a few more in some cases, the number 
depending upon the size of the sporangium. 
In one case observed, where the zoospores seemed to be rather 
sluggish in their development and the movements not so active, the 
form was not so pronouncedly reniform, but more nearly oval, and 
after separating very little active movement took place, the individuals 
soon rounding off as they do when ready to germinate. Two such 
zoospores soon came in.contact and immediately fused into a larger 
one, the fusion taking place in about ten seconds. The further fate 
of these zoospores was not followed. This fusion may be due to the 
peculiar conditions of environment, perhaps to the want of fresh 
water in the limits of the cell culture. From some observations made 
on the developing zoospores under similar conditions, it appears 
possible that it may be due to the variations of tension existing between 
the individuals and the original mass of protoplasm, the tension of 
the entire mass being directed toward keeping the mass intact and 
the tension of the individuals tending to separate the masses into 
smaller individuals. Two cases were observed which had progressed 
to some extent toward the formation of the zoospores. In one cas¢ 
there were two zoospores forming and in the other case four zoospores 
were forming from the spherical protoplasmic mass, which shad 
collected at the end of the exit tube after passing from the sporang!um- 
In each case the zoospores were about one-third formed. The 
preparation had been in the cell culture for two days and the water 
had been replenished a few times, as it had partially evaporated, by 
running fresh water under the cover from the edge, the cell cultures 
being made simply between the cover glass and the glass slip and net 
in a ring-cell, or vAN TreGHEM cell. The oxygen thus accessible 
to the organism was very small, though this did not seem to hinder the 
development during study, if water were added every few moments, 
as would be necessary when the preparation was not protected in @ 
