334 BOTANICAL GAZEITE ~*~ [NOVEMBER 
by Hartoc’® (p. 216) in the case of the escaping zoospores of 
Achlya and Aphanomyces. 
In some of the cell cultures in which numerous zoospores of 
Lagenidium were developing, there were several sun animalcules 
(Actinophrys sol). Some of these were so full of zoospores which 
they had caught, that they appeared to be a rounded collection of the 
zoospores, the convex outer portion of the zoospore only showing, 
and the entire surface of the Actinophrys appearing like a piece of 
mosaic made up of these unfortunate creatures. The delicate arms — 
or rays of the Actinophrys radiated for a distance of 20-30 #, and in a 
large number of cases which were observed, whenever a zoospore 
passed within reach of these rays, no matter how swiftly it was moving, 
it was suddenly paralyzed and halted; then the reniform shape 
gradually became spherical and it was slowly drawn by “invisible 
threads” to the body of the Actinophrys and slowly wedged its way 
between those which had preceded it. 
LAGENIDIUM AMERICANUM 
Another species of Lagenidium, which seems to differ from any 
described, was found in various species of Spirogyra (5. varians, S. 
calospora, and S. insignis) collected in a pool beyond Forest Home, 
April 11, 1895. It is parasitic in the zygospores and conjugating 
cells of the spirogyras, but has not been observed to develop in the 
vegetative cells... The vegetative phase of the fungus consists of 
strongly curved and coiled tubes, 4-8 » in diameter, the size varying 
so that the contour and diameter of the threads is very irregular. 
It is profusely branched, and has also numerous short branches. It 
frequently fills the zygospores so completely that the individual course 
of the thread can be followed for only a short distance. At maturity — 
the tube, as in the other species, is separated into segments of various 
lengths by the development of cross-walls, forming the zoosporang!a. 
Frequently there is a constriction at the septum. Numerous Z200Sp0" 
rangia are developed in a single zygospore. The exit tubes are slender, 
measuring about 2 « in diameter, and of variable length according to 
the direction in which they emerge from the zygospore and the wall — 
o Hartoc, M. M., Recent researches on the Saprolegnieae. Annals of Botany 
2:201-216. 1888. 
