344 BOTANICAL GAZETTE [NOVEMBER 
bles the daughter stars formed in mitoses where the chromosomes 
themselves are elongated. These fibers may be used to determine the 
chromosome number, since each one of them is connected with a 
chromosome. This can be done more easily in this stage than in 
metaphase, because the fibers are larger than the chromosomes and 
because they spread apart as they separate, sometimes giving views 
of all four at once, while in any other stage the full number can be 
seen only from polar view (jig. 16). 
These radiating bands of fibers are now drawn in, leaving the 
two poles connected only by a central band (figs. 20, 22), which 
becomes considerably attenuated as the two chromatic masses move 
farther and farther apart. The daughter nuclei become separated 
by distances considerably greater than the original length of the 
spindle. In moving apart one or both of them frequently swerves 
from the axis of the spindle, so that the connecting fibers meet at a 
considerable angle (fig. 33). As was indicated in the paper on the 
reconstruction of the nucleus (Griccs 4), this habit makes it almost 
impossible to associate the daughter nuclei in pairs after the wisps 
of spindle which point to the original position of the equator have 
disappeared, especially since the wide separation greatly lessens the 
chances of securing both in the same section. For this reason all 
figures of stages after this period are drawn from only one of the 
daughter nuclei. After the spindles have broken in two, the telo- 
phases are very inconspicuous objects; though they stain deeply, the 
chromatin is concentrated into so narrow a space that they are 
practically invisible under any but the highest power (figs. 23, 35)- 
The aster 
As previously reported by Stevens, Kusano, and myself, there 
are no granules, radiations, condensations, or any other indications 
of the presence of centrosomes at the poles of the spindle during 
prophase or metaphase. But about the time the two halves of the 
spindle separate, radiations begin to emanate from the chromatic 
masses. At first so delicate as to be on the very limit of visibility, 
the aster rapidly increases in prominence until it becomes exceedingly 
conspicuous, clearly visible under a magnification of forty or fifty 
diameters. At first the radiations may appear to emanate from the 
