384 BOTANICAL GAZETTE [NOVEMBER 
at the poles. After the organization of daughter nuclei the central 
spindle disappears, and the formation of a cell plate at the expense 
of the cytoplasm begins only after the nuclei have grown to a consider- 
able size. During the second and ensuing divisions the same number 
of the chromosomes is present. 
Thus the sporeling from the fertilized gametes of Culleria multifida 
develops into a structure of the Aglaozonia form of this species, which 
contains 48 chromosomes. 
Aglaozonia reptans 
ZOOSPOROGENESIS 
The forms which evidently fall under the category of this species 
show somewhat wide variability in their habit. The mitosis in the 
vegetative cells of the form was studied. Since the essential features 
of the division are similar to those of Cutléria, detailed accounts will 
be omitted at this time. The fundamental difference between the 
two forms is that the nucleus of Aglaozonia contains 48 chromosomes, 
the number persisting up to the formation of the zoosporangium. 
Zoosporangia are produced on the upper surface of the thallus. 
The origin of the structure is as follows: A superficial cell of the 
thallus slightly elongates and divides, giving rise to two cells, the 
upper one of which becomes as a rule a zoospore mother cell; not 
infrequently, however, several cell divisions take place, and in that 
case the terminal cell becomes the mother cell. The growth of the 
zoospore mother cell is striking; it elongates until its length becomes 
three to six times its width. The elongation of the cell is always 
accompanied by the growth of the nucleus, which remains in the 
middle region of the cell. 
When the nucleus is approaching the prophase, the chromatin 
network, by a possible rearrangement of the material, becomes ie 
and less branched, and finally there results a tangled mass of continu- 
ous threads traversing the nuclear cavity. The tangled threads, 
becoming more and more uniform in thickness, become transformed 
into regularly arranged loops centering at a certain part of the cavity, 
which represents the beginning of the synapsis stage. The synaptic 
accumulation of the threads occurs always in association with the 
