1909] YAMANOUCHI—CUTLERIA AND AGLAOZONIA 385 
kinoplasmic mass outside the nucleus, but its relation to the axis of 
the cell is varied. 
The loops shorten and thicken, and finally they break up into 24 
bivalent chromosomes, each derived from one of the loops. The 
heterotypic figure thus establishéd is intranuclear, and the axis of the 
spindle is in various directions. Between the first and second divi- 
sions the daughter nuclei rest. The four nuclei which are products 
of the second division contain 24 chromosomes, and the same number 
is found in the third division which gives rise to eight nuclei. 
When the zoospore mother cell has reached the eight-nucleate 
Stage, there occurs generally a cleavage of the cytoplasm, which 
divides the whole contents of the mother cell into eight’ zoospore prim- 
ordia (Anlagen). Not infrequently, however, one or two more — 
divisions occur after the third, and as a consequence there are pro- 
duced 16 or 32 nuclei, and in those cases 16 or 32 zoospores are 
formed. 
The nuclear divisions in the mother cell, as well as the segmenta- 
tion of the zoospore primordia, always occur simultaneously. As was 
stated before, the chromosomes contained in the thallus of Aglaozonia 
are reduced to one-half during the first two divisions in the zoospore 
mother cell, and 24 chromosomes are involved in the zoospores. 
The zoospore germinates independently, without any conjugation; 
Possibly 24 chromosomes, the reduced number, may persist in the 
Structure arising from the germination of the sporelings of the zoo- 
spores, but the nuclear details in the sporelings have not yet been 
completely investigated. 
Summary — 
The nuclear conditions during the life-history of Cudleria multifida 
and A glaozonia reptans may be summarized as follows: 
1. The nucleus of both male and female plants of Cuéleria multi- 
fida contains 24 chromosomes; and the male and female gametes 
Produced contain the same number. 
2. In the union of gametes the number is doubled, and 48 chromo- 
Somes appear in the sporelings, which develop into the Aglaozonia 
form of Cutleria. Therefore it is evident that the individual bearing 
the name of Cutleria multifida represents the gametophytic phase of 
