NO. 2 RECOGNITION AMONG INSECTS — McINDOO 7 



from a frame in hive No. 5a. One of these 10 bees was put into each 

 case ; all 10 were attacked quite forcibly. This proves that the bees 

 from these two hives had become enemies. 



To determine if these sister bees, which had become enemies by- 

 having their original colony divided, could be made friends again, 

 ten cases similar to those already described were used. These cases, 

 however, were an inch deep and wire screen served as tops and 

 bottoms. These cases were numbered i to 10 and may hereafter 

 be known as the wire-screen cases. 



Fifty middle-aged workers were removed from a frame in hive 

 No. 5a and were placed in each of three of these cases (Nos. i to 3). 

 Fifty workers from a frame in hive No. 5b were likewise placed in 

 each of three more of these cases (Nos. 4 to 6). As controls 50 

 middle-aged workers from a frame in hive No. 29 were placed in 

 each of two more of these cases (Nos. 7 and 8), and in a like manner 

 50 workers from hive No. 49 were placed in two more of these 

 cases (Nos. 9 and 10). A large lump of queen-cage candy (made by 

 kneading confectioner's sugar in a small amount of honey) was kept 

 continually in each case. A piece of cotton wet with water was also 

 constantly kept in each case. The cases containing the bees from hive 

 No. 5a, one of those containing bees from hive No. 29 and one of those 

 with bees from hive No. 49 were kept in the south room of the 

 laboratory, while the other five cases were kept in the north room of 

 the laboratory. The apices and bases of these cases rested on supports 

 above rigid tables by open windows so that the air could pass freely 

 through the cases. 



Three days later a worker from each of cases Nos. 4 to 6 was put 

 into each of cases Nos. i to 3 ; likewise, a worker from each of cases 

 Nos. I to 3 was put into each of cases Nos. 4 to 6. All six introduced 

 bees were received peacefully without even the least signs of hostility. 

 This proves that these sister bees had become friends again, provided 

 they responded normally. To test this possibility, 15 minutes later 

 a worker from hive No. 23 was put into each of the six cases ; all 

 six introduced bees were attacked quite forcibly. After another inter- 

 val of 15 minutes a worker from a frame in hive No. 5a was put into 

 each of the six cases; all six introduced bees were attacked quite 

 forcibly. Fifteen minutes later a worker from a frame in hive 

 No. 5b was put into each case ; as usual, all six introduced bees were 

 attacked quite forcibly. An hour later still workers from cases 

 Nos. 7 to 10 were put into these six cases : all six introduced alien 

 bees were attacked only lightly. 



