8 SMITHSONIAN MISCELLANEOUS COLLECTIONS VOL. DO 



Concluding from the foregoing experiments, the following con- 

 ditions are indicated: Hive No. 5 had a hive odor and after this 

 colony had been divided eight days each new colony thus produced 

 had formed a new hive odor different from the hive odor of the other 

 new colony. The workers of any colony carry the hive odor formed 

 by their own colony. After a confinement of three days in the wire- 

 screen cases, workers do not produce a new hive odor, but lose the 

 hive odor that they bore when removed from their hive. That they 

 are not able to form a new hive odor in these cases and that they 

 lose the hive odor carried by them is probably due to air constantly 

 passing through the cases. The workers, therefore, from hives 

 Nos. 5a and 5b confined in cases Nos. i to 6 became friends again 

 because their respective hive odors had disappeared, and they failed 

 to attack each other even lightly because they were offspring from 

 the same mother. These workers fought those from cases Nos. 7 

 to 10 only slightly because the latter had lost their respective hive 

 odors and the slight hostility exhibited was due to the fact that the 

 bees in cases Nos. 7 to 10 were offspring from different queens. 



The foregoing experiments were repeated three times with bees 

 from hives Nos. 5a and 5b. Each of the three lots of bees was put 

 into cases Nos. i to 6 as already described for lot No. i in the pre- 

 ceding experiments and workers used as controls from different 

 hives were put into cases Nos. 7 to 10 as before described. After 

 a confinement of three days in the cases all of these workers were 

 tested. Of the 18 workers tested one at a time from hives Nos. 5a 

 and 5b, 16 were received peacefully without the least signs of hos- 

 tility ; the bees showed slight signs of hostility toward the other two 

 but did not attack them. On the fourth day after confinement 18 

 more workers were tested one at a time; all 18 were received peace- 

 fully without signs of hostihty. After a confinement of only one 

 day in these wire-screen cases and when tested one at a time, each 

 introduced bee was attacked only lightly. The workers used as 

 controls in all of these experiments were tested and the results were 

 always the same as described for the first lot. 



Hive No. 73 was equally divided on October 15, making two new 

 colonies, Nos. 73a and 73b. The brood, honey, and pollen were also 

 divided as equally as possible and the queen was left in the hive on 

 the old stand. Five months previously this colony had been united 

 whereby strange bees from another queen were mixed with those 

 already in this hive. By October 15, all the bees added by uniting 

 were certainly dead. Three days after the colony had been divided, 



