12 SMITHSONIAN MISCELLANEOUS COLLECTIONS VOL. 68 



No. 28 were put into a queen cage and the cage with bees was 

 immersed in water for 15 minutes. The apparently Hfeless bees 

 were then removed from the cage and were allowed to become dry. 

 When dry and when they could walk and fly normally, one of them 

 was introduced into each of the 10 cases. This set of experiments 

 was repeated seven times. Of the 80 immersed workers tested, 40 

 were attacked more or less and 40 were received peacefully, although 

 the bees seemed to regard some of the latter also as strangers. As 

 a control the bees placed in the cases were taken from a frame of 

 hive No. 25, and 10 guards from the entrance of the same hive were 

 immersed in water for 15 minutes. When dry one of the immersed 

 guards was introduced into each case ; six of them were attacked 

 lightly and four were received peacefully, although they were cleaned 

 roughly. 



Workers immersed in 5 per cent alcohol for one minute and then 

 in water for 14 minutes were invariably attacked when introduced 

 among strange bees ; in all 30 such workers were tested. 



Workers from a frame of hive No. 36 were put into the cases and 

 10 workers from the entrance of the same hive were immersed in 10 

 per cent alcohol for five minutes and then in water for 10 minutes. 

 When these immersed bees were tested all were attacked. 



Twenty workers from each of hives Nos. 15 and 17 were placed 

 in the queen cages. They were immersed in 10 per cent alcohol for 

 five minutes and then in water for 10 minutes. When dry each lot was 

 put into a case, and later when mixed they were still hostile to each 

 other. This set of experiments was repeated by taking two lots of 

 bees from the entrance of the same hive. This time the bees were 

 immersed in 10 per cent alcohol for 10 minutes and then in water 

 for 10 minutes ; all 40 revived as usual. When mixed they were not 

 hostile to each other, but when strange workers were introduced, the 

 strangers were attacked. The immersed bees also attacked hive mates 

 taken from the entrance of their own hive. This experiment was 

 repeated by immersing 200 bees from the entrance of hive No. 56. 

 These workers were later quite hostile to strange bees as well as to 

 hive mates. 



Fifty workers from a frame each of hives 38a and 38b were 

 immersed in each of 10 per cent alcohol and water for 10 minutes. 

 When dry each lot of bees was placed in a wire-screen case. A 

 hive mate that had been confined with others in a wire-screen case 

 for four days and that by test had lost its hive odor, was placed in 

 each case of immersed bees. Each introduced bee was attacked quite 



