14 SMITHSONIAN MISCELLANEOUS COLLECTIONS VOL. 68 



forceps. The bees were then gently removed from their cells and 30 

 were placed in each of the two cases (Nos. i and 2), and a piece 

 of candy and a piece of cotton wet with water were also put into 

 each case. Six days later a worker was taken from case No. i and 

 was put into case No. 2 ; then a worker from case No. 2 and was put 

 into case No. i ; both introduced workers were attacked lightly. 

 After an interval of five minutes the following controls were used: 

 Two sister workers just removed from sealed cells of hive No. 60 

 were put into these cases-; both were attacked slightly. Five minutes 

 later a fanner from the entrance of hive No. 60 was put into each 

 case; each introduced fanner was attacked lightly. Five minutes 

 later still a fanner from the entrance of hive No. 19 was put into 

 each case; again each fanner was attacked slightly. After a con- 

 finement of nine days in these cases the young workers were quite 

 hostile to each other. 



That a lot of isolated sister bees can not form a new hive odor 

 unless confined in a container with close-fitting walls is evident, 

 because the sister bees in wire-screen cases Nos. i to 3 were often 

 tested in the same manner as described above after a confinement of 

 several days. They never showed signs of hostility, and the same 

 may be said about the sister bees in wire-screen cases Nos. 4 to 6, 

 and about those in cases Nos. 7 and 8, and also about those in cases 

 Nos. 9 and 10. 



The foregoing experiments were repeated by using workers 21 

 days old, when just ready to emerge from sealed cells. They were 

 confined in a wire-screen case for 21 days. On the first day after 

 being put into the close-fitting cases Nos. i and 2, they were slightly 

 hostile to one another ; on the fourth day they were considerably 

 hostile and on the sixth day they attacked one another still more. 



These experiments were repeated by using workers bearing a 

 hive odor. One hundred middle-aged workers, all from the same 

 frame in hive No. 49, were put equally into the two close-fitting cases 

 Nos. I and 2. After a confinement of three days in these cases they 

 were tested in the usual manner ; the bees attempted to attack both 

 introduced sister workers. One hundred more sister bees from the 

 same hive were used in the same manner; on the third day when 

 tested, one introduced bee was attacked quite forcibly and one was 

 attacked lightly. One hundred sister workers from hive No. 56 were 

 likewise used ; on the third day of confinement when tested, they 

 showed no signs of hostility toward one introduced bee, but attempted 

 to attack the other one ; on the fourth day they were slightly hostile 



