l6 SMITHSONIAN MISCELLANEOUS COLLECTIONS VOL. 68 



then the frame with comb was put into one of the containers. Next, 

 a half frame of bees from hive No. 8i was shaken into each container, 

 the Hd of the container was then securely fastened and all four con- 

 tainers were placed on hive bodies inside the laboratory by an open 

 window so that the wind could blow through the cages. 



Four days later 20 workers from cage No. i were put into each of 

 five glass observation cases already described, and then one worker 

 from cage No. 2 was put into each of these five cases. Twenty 

 workers from cage No. 2 were put into each of five other glass obser- 

 vation cases, and then one worker from cage No. i was introduced 

 into each of these five cases. No signs of hostility were shown toward 

 any one of the 10 introduced workers. A few minutes later a sister 

 worker from a frame in hive No. 81 was put into each of the 10 cases. 

 The bees showed no signs of hostility toward one introduced worker ; 

 they lightly attacked seven ; and two were attacked quite forcibly. 

 After confining the bees in the cages seven days, the experiments 

 were repeated and the same results were obtained. A few minutes 

 later sister bees from boxes Nos. i and 2 were put into the cases. 

 Eight of the introduced workers were attacked lightly and two were 

 attacked considerably. 



After confining the bees seven days in the queen-mating boxes, 

 they were tested in the same manner as described for the bees from 

 the cages. Of the 10 workers introduced, four were attacked lightly 

 and six were attacked considerably. 



After confining bees in these boxes and cages seven days they 

 become more or less black and shiny and the abdomens are much dis- 

 tended with waste matter in the intestines. They are less active than 

 usual and are perhaps more or less abnormal in some ways but after 

 being confined seven to ten days, they seem to respond to odors 

 normally. 



All of the foregoing experiments were repeated by using bees 

 and comb from hive No. 67. In these experiments, however, 20 

 workers from one cage were placed in each of the 10 cases and a 

 worker from the other cage was introduced a few minutes later into 

 each case. Thus for the cages, 20 workers, one at a time were put 

 into the cases and all of them were received without any signs of 

 hostility. The same procedure for the boxes was followed, and of 

 the 20 workers introduced, the bees attempted to attack two ; 10 

 were attacked lightly ; and eight were attacked quite forcibly. When 

 several of the workers from the boxes were placed on the alighting 

 board of their own hive (No. 67), some of them were attacked by 



