1 6 Maurice Croivther Hall 



grown gregarines. Quite commonly the satellite is smaller than 

 the primite, but I have never found the reverse true. Lines of 

 individuals may be readily resolved into the separate pairs of 

 conjugates. 



I occasionally find a gregarine in which the protomerite shows 

 an anterior peripheral circlet. On breaking up a recently formed 

 conjugation I have found that the protomerite of the satellite 

 showed the same thing, indicating that the circlet in gregarines is 

 probably due to the breaking up of a conjugation. Biitschli 

 (1882) refers a similar observation to the same cause. Whether 

 conjugations are broken up in nature or are broken in teasing the 

 host intestine is not certain. In a solidly-formed conjugation the 

 protomerite of the satellite undergoes a great reduction and at 

 times shows only as a thickened band (fig. 7). 



An interesting thing is the fact that often there is seen in a 

 newly-formed conjugation a highly colored orange or orange- 

 red spot at the point of contact either in the deutomerite of the 

 primite or the protomerite of the satellite. This may be an indi- 

 cation of a substance of assistance in forming the fusion. 



Cysts may be obtained from the intestine or the caeca or from 

 the feces. In encyst ment gregarine conjugates bend at the point 

 of contact, as Biitschli (1881) first noted, so that the two come 

 to lie side by side, the protomerite of one opposite the end of the 

 deutomerite of the other and in contact with it. The surfaces in 

 contact flatten out, the conjugation assuming a shape varying 

 from spherical to a much elongated oblate-spheroidal, of which 

 each individual forms a symmetrical half. I have not observed 

 the rotation during cyst formation noted by Biitschli (1881) and 

 Crawley (1905). Conjugations of individuals of diflferent size, 

 so far as I have observed, do not show as solid, final afifairs as do 

 those where the individuals are of approximately equal size, and 

 I am inclined to think with Marshall (1893) that they do not 

 lead to successful encystment, at least while the size remains 

 dififerent. 



That conjugations, where the individuals are both small or both 

 large, may successfully encyst is shown by the variation in size 

 of the resultant cysts. The cyst usually shows outside the limit- 



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