132 BOTANICAL GAZETTE [ FEBRUARY 
From drop cultures it was ascertained that germination might 
begin in a few hours ; and in twenty-four hours there was considerable 
germination and growth about the spores. Again, at a uniform tem- 
perature, in two and one-half days, considerable aerial mycelium was 
developed, but rarely any spores, and this stage of growth was taken 
as the age limit for tests upon mycelium. (See table VI. 
For the tests with dry spores, as in table VII, fresh spores were 
exposed on glass slips, and then inoculations were made immed 
therefrom. 
TABLE. ¥: 
35° CG. AGAR CULTURES. 
es 
| When exposed, | Length of ti : 
Exp. no. ce seg ak : ME’! Successive days | Result 
I Immediately | 6 hours Good growth 
2 “ puree One center 
5 Continuously No growth 
4 6 hours 24 hours Good growth 
§ 2 ‘6 2 ‘“ Good growll 
6 Immediately) 6 “ 2 Good growth 
7 . 6 * 4 Good growth 
quite effectually inhibits growth, when immediately exposed to ! | 
temperature (see exp. 2); while a few hours after germination mo 
have begun, the same did not hold true. : 
TABLE II. 
° 
: C. AGAR CULTURES. 
When exposed, Length of time ; ult 
Exp. no, dike: ‘soting enue Successive days | Res 
ce Sa mae 
8 Immediately | 6 hours Good gr om 
9 “ee 9 ““ Good growt 
10. «14g « Good growth 
s a e No grow 
12 = Continuously} One week No sone 
13 6 hours 24 hours seis th 
14 cP ee Goos ar ewth 
15 | 24 “ 24 “ Good gro’ 
The results at 37-7° C. repeat and confirm those of table I, ip ‘i tit 
freshly sown spores are readily killed in twenty-four hours, whi 
