Noteworthy anatomical and physiological researches. 
Researches on transpiration and assimilation.’ 
I. Transpiration experiments. 
Stahl’s purpose in writing the paper here reviewed was 
mainly to present a method by means of which it could be 
demonstrated to the eye whether a plant loses water by trans- 
piration, and through what parts of its surface the loss takes 
place. The method does not take the place of weighing the 
loss of water for all the more accurate experiments. It is val- 
uable on account of its simplicity and the facility it offers for 
public demonstration coupled with the fact that by it certain 
problems can be investigated which are not possible by other 
methods. 
Marget? was probably the first investigator who used the 
method about to be described. According to Stahl he im- 
pregnated white paper with a mixture of mercurous chloride 
and pallidous chloride, and also with pallidous chloride, tat 
taric acid and ferrous chloride. In the dry state the pape! 
was whitish-yellow, but as it absorbed water it became darker 
and finally black. When applied to the transpiring surface 
the loss of water could be estimated by the change in color. 
The color could be fixed at any point by wetting the pape! is 
ferric chloride.? Others who have tried to use Marget® 
1 Stahl, Ernest. Einige Versuche iiber Transpiration und Assimilation. Bot. 
Zeit. 652: 117-145. p/. 4. Jl. 1894. 
2 Marget, Sur les fonctions des feuilles. R6dle des stomates dans l’exba 
lation et dans l’inhalation des vapeurs aqueuses par les feuilles. Comptes-re® 
dus de l’acad. des sc. 87: 293. 1878. 
echerches sur la transpiration des végétaux et le réle des feuilles dans @ 
phénoméne. Annales de la soc. d’Agric. etc. de Lyon 1878: Lxxv-Lxxvul. 
® The first account of this method published by Marget in Annales de la a 
d’ Agric. etc. de Lyon 1. c. says that the paper was treated with a solution 
pallidous chloride, tartaric acid and ferric chloride, mot ferrous chloride. The 
aper was then dried and exposed to light which changed the ferric chloride © 
ferrous chloride. In this condition the paper when moistened turned darkef 
b as amount of moisture increased. 
Comptes rendus 1. c., he simply says that he used a paper sensitized photochem, 
i i i i ide. The method 
of doing this is not given but is probably the same as that given in the 
EY i am 
oppor 
tages over the cobalt method if it could be used readily. I find no mention ® 
the papers cited of the use of mercurous chloride. 
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