1899] _ STRUCTURE OF THE EMBRYO-SAC 155 
the ovule in S. eurycarpum Engelm. and was struck with certain 
peculiarities which it exhibited, some of which were referred to 
and figured in my paper on Naias and Zannichellia. Most 
noticeable was the unusual development of the antipodal cells. 
The study of Sparganium was interrupted for various reasons, 
but in the meantime material of other species was collected and 
the subject taken up again. During the past summer a good 
supply of S. simplex Huds. was obtained from Tallac, on Lake 
Tahoe, and through the kindness of Miss Alice Eastwood, a 
quantity of the Californian species, S. Greenii Morong, collected 
at Lake Merced near San Francisco; was sent me. I had alsoa 
small amount of a Japanese species, S. dongifolium Turcz., collected 
by me at Akkeshi on the island of Yezo. Of these, S. simplex 
Proved the most satisfactory for study and these preliminary 
notes refer principally to that species. 
All the Species of Sparganium are moncecious, the heads of 
pistillate flowers being at the base of the inflorescence. There 
- mally three or four of these pistillate heads in S. semplex. 
alae flower in this species consists of a single carpel, 
eit ead ovule, and surrounded by a number of mem- 
Me Oral leaves. In SS. eurycarpum and S. Greenii, the 
out A composed of two carpels. 5 
gor sections of the carpels for the study of the 
Niranjies Sg in the youngest stages, care must be taken 
tration of the be of the hard outer tissues to permit the pene- 
ieee xing and embedding agents. Of the various fix- 
‘mployed, the best results were obtained from the 
in alcohol, Th ania pf corrosive sublimate or picric acid 
A double = ee acid mixtures proved less satistactory: 
best rea, ismarck-brown and anilin-safranin gave the 
All of m 
the origin of 
© develo 
and fro 
Y material was too old to make it possible to study 
the ovule and the early history of the embryo-sac. 
ap aay : the flowers has been studied by Dietz,? 
*Biblioth sures it looks as if in Sparganium, as in most of 
ca Botanica 5i—. 188%, 
