EFFECT OF ETHER UPON GERMINATION 463 
hich spores of Mucor and Penicillium were used. For these 
“experiments fresh spores were placed upon a mixture of 5 per 
nt. gelatin and 10 per cent. sugar, and these preparations 
ere then placed in damp chambers, containing respectively 0.1, 
> 1,2.5,5, and 10° of ether, one chamber remaining free from 
_ ¢ther for control. 
TABLES V,. VI. SPORES. 
Ss placed on a medium of gelatin 5 per cent., sugar 10 percent. Temp. 18-20°C. 
V. Mucor. 
No. hours from ee 
No. ho Total durat | 
Chae ce qitired for ger a Ae of eee Appearance of mycelia 
minatio new spores (hours) 
Sl | 
i4 | 24 38 Branched 
f 14 24 38 Short and blunt 
*-5 24 24 484 ‘Blunt at ee ce elong’d 
5: 36 24 6 | Elonga 
*240 No_ spores 288 | Long and Saale 
a VI, Penicillium. 
9. 12 28 40 Branched 
sats 12 28 40 
I, 12 28 40 Short 
25 24 24 48 
- S. 36 36° 72 Peng kad ancy 
a7 216 $264 faio a long and branc 
“*lfat end of 240 hours spores were placed on the nutritive medium in an ether- 
atmosphere the spores germinated in 14 hours, and in 24 hours a few spore cases 
Spores had formed. 
/9Pores in this stage if removed and Lames: in an ether-free sno ddd aka! 
and produced new spores in 40 hou 
these new spores hah nied poe on ve nutritive medium they anee in 
time (12 hours) and produced fruit in 40 hours. The characteristic blue co 
Present, ' 
0.1° of ether seemed in some instances to accelerate 
tion slightly. This point was somewhat difficult to 
ermine however, since the normal period of germination does 
pereed fourteen hours under the conditions used. : 
nthe damp chamber containing 1° of ether, many of the 
