1896.] Cultures of Exobasidia. 105 
fusiform secondary spores, such as Woronin!® and Bre- 
feld’* figure, which kept on multiplying for some time and 
then finally refused togrow any more. Transfers of these on 
live plants produced no results, sothe plan was abandoned for 
the time being and attention was turned to making {nocula- 
tions on living plants from fresh material. 
For the cultures on live plants healthy young specimens of 
Andromeda ligustrina, Rhododendron viscosum, and Gaylus- 
saccia resinosa entirely free from any signs of Exobasidia were 
selected. The experiments were carried on at Newport, 
R. L, during the summer of 1894, the plants having been se- 
lected the previous year so that they would be well established. 
During the course of the experiments the plants were kept 
ina glass case excluded from accidental infection and sep- 
arated from each other. Beginning on the roth of July, and 
or a number of days following, infections were made on these 
plants with the spores of various Exobasidia. 
he specimens of Exobasidium used were selected with 
great care, only perfectly fresh ones which were as far as pos- 
sible removed from contamination with other spores being col- 
ahi As soon as picked they were placed in sterilized tin 
0 
cde the surface of the distortion being scraped with a ster- 
llized Platinum spatula moistened with a drop or two of 
_ The spores thus obtained were examined in a 
Sterilized Van Tieghem cell and afterwards transferred to the 
ae and young leaves of the plants to be inoculated. In 
a. let several plants of Andromeda, Rhododendron, and 
ied ussaccia of the species already named were infected. The 
Plants thus Prepared were then isolated in a moist chamber to 
“wait developments. This treatment was repeated on other 
Specimens, 
ie the Same way other plants of the same kind were inocu- 
late with spores of Exobasidium Vaccinii and also duly iso- 
eats The €xperiments on Rhododendron viscosum failed for 
; T€ason or other and we will turn our attention to those 
ont : 
he other plants which were more successful. 
19 
11 
lc. pf 
- 3. figs. 10-13, 
Untersuch, aus d. Gesammtgeb. der Myk. 8: 9. p/. 1. figs. 17-22. 
