110 The Botanical Gazette. fMareb, 
fuse together, beginning at the carpogonium end and there 
results a large ‘‘fusion cell.” 
In Chylocladia and Champia the fusion of the cells of the , 
coalesce. a 
In Champia parvula the hook-shaped process from the fusio! 
cell, that is put out towards the auxiliary cell, contains ¢ | 
single large nucleus, 
(4) The wall of the cystocarp is formed in the same mal | 
growth and branching the wall of the cystocarp is formes 
a loose network of filaments that are pushed against the “ 
of the sporangium wall by the development of the glomerw® | 
of spores, 
The material with which the writer worked was collecteé | 
at Woods Hole, Mass. Theo 
servations were made chiefly from sections, as it was not ea F 
2 crush out Preparations, particularly of older stages of | 
ruit. If care be taken the tissues of the plant may bet 
of certain stage The writer also employed a method ¢ i 
sectioning suggested by Mr. W. J. V. Osterhout, in wht 
€ tissues were fr i ; pe 
isfactory and atime-saver. The specimens for the most P 
were stained zx toto with Mayer's acid haemalum. 
