1896, Anatomical and Physiological Researches. 291 
As to the influence of CO iol agi 
excretion of oxygen, most weit ie premiersnjesieass. and the 
Shots a 
age diminishes it, or even works inj 
But carbon-assimilation d s injury to the plant. 
: epends on the function of ch 
phyll, and this points to t of chloro- 
not provided with ie a ngs ae ree ae plants “se 
centage of CO,; for acco i ey could: not bear this per- 
chlorophyll is Sead t rding to Boehm, the formation of 
with 20% CO,. in air with 2% and suppressed in air 
As to the inf 
susceptibility tb Sel di eigher 3 phenomena of movements, 
along stay (6~12 hours) in CO, Prades isi er 
alive irritability return . + Solna 
toatmospheric air. I s when the plants are transferred again 
an accommodation t 2 -~ sleep-movements of Oxalis leaves 
The plasma-streami il . CO,-atmosphere is even possible. 
C0, it begins Soa Gh sya: an etal Stop ped by 
byair. The motilit Hass me Ce if the CO, is replaced 
the Backs. tco | ae of protoplasm is not destroyed unless 
Lopri : : 
in ec erttook to re-investigate the action of pure CO, 
toplasm witho ape prepara ne upon the vital activity of pro- 
living plant ¢ * chlorophyll, and especially upon the growth of 
cautions Oe In the preparation of gases special pre- 
ated by the hati tohave them pure. The CO, was pre- 
Which yields y process of heating potassic bicarbonate, 
Pores first ete best conditions only half its gas—a 
Laure h sed in physiological work by Schloesing and 
Pr of HC] Tahitian of obtaining CO, free from va- 
Marble deter when this acid is used to liberate the gas from 
| The difficult mined the author to avoid this common process. 
 8Ypsum tals 4 refilling Kipp’s apparatus on account of the 
| Nordinari} ed when H,SO, is used, although it yields ex- 
Method, f pure CO,, deterred him from using Bunsen’s 
— Mthod oh ae 
| Dire and s we potassic bicarbonate used must be chemically 
| ad nitrates Clally tested as to its freedom from ammoniates 
the oxy 
tlorate eon was prepared by heating the purest potassic 
BN was at - glass retort with the usual precautions. Hydro- 
| Silfuric acid tst prepared in a Kipp’s apparatus from zinc and 
and washed through plumbic acetate and KOH, 
| Ut ater 
Compressed H was purchased and purified. 
