162 BOTANICAL GAZETTE [SEPTEMBER 
tendency for both derivatives of the primary spermatogenous cell to 
function as body cells. 
The material for the present study was secured through the kind- 
ness of Dr. L. Cockayne of Christchurch, New Zealand, and Miss 
INEz FRANCES STEBBINS of the Huguenot College at Wellington, 
Cape Colony, South Africa. To both are due our thanks for their 
kindness in obtaining the material at no inconsiderable personal 
inconvenience. Though the collections were necessarily few, yet 
they include a considerable range of developmental stages in each 
of the species. The material was fixed in a 5 per cent. solution of 
commercial formalin in 70 per cent. alcohol. The fixation has proved 
unexpectedly good, except for stages from the rounding-up of the 
mother cells to the time when the microspores have a good firm wall 
and abundant contents. The shrinkage and general distortion within 
the limits just mentioned have rendered necessary the omission of a 
number of what appear to be rather interesting and perhaps impor 
tant details. Some of these will be mentioned briefly in the general 
account. It is hoped that other material so killed and fixed as to 
show details of cytological structure will be in hand within the neat 
future. The preparations have been imbedded in 54° parafim, Es 
3-5 #, and stained in Haidenhain’s iron alum hematoxylin stall, 
alone or counterstained with various aqueous stains such as orange 
G, Bismarck brown, erythrosin, etc., and with the saffranin gential- 
violet combination. Three species have been examined, though 
not covering exactly the same ground. vate 
Observations 
The cones of P: totarra Hallii are of a generally oval — 
varying from 3 or 4 to 15 or 20™™ in length, and from 2 to 4” 8 
diameter. They are usually in pairs, one being large and one a 
The base of the cones is surrounded by several closely appress* 
rigid, scalelike bracts. The peduncle is quite short or 4 
lacking. The cones of P. nivalis are somewhat shorter, not Paz’™s 
and decidedly slenderer. The cones of the third species (the a 
of which was lost in transit) are similar in shape to the first = 
are smaller. In other respects the cones are very similar. all 
totarra is one of the tallest of forest trees and P. nivalis a very S™ 
