172 BOTANICAL GAZETTE [SEPTEMBER 
whether the two cells are body cells or male cells. If the third suppo- 
sition is true then there are four prothallial nuclei, a generative cell, and 
the tube nucleus. 
In a number of preparations there seemed to be a cell on the side 
of the body cell opposite the stalk cell (fig. 27), but in no case could 
I satisfy myself of the presence of a nucleus; though in view of the 
figures published by JEFFREY and CHRYSLER it was diligently looked 
for. In fig. 27 is shown a mass at one end of this “‘cell,’’ which may 
stand for a degenerating nucleus, although I think not. . It is very 
indistinct and may very well be a mere aggregation of slightly denser 
cytoplasm. By constructing a model of the cell complex, where 
the body cell has sunk down between the cells of the second tier of 
the prothallus, and then cutting it so that the section will pass about 
centrally through stalk and body cell and include most of one-half 
of them and take a slice off the prothallial cells lying beyond them, 
it is possible to get preparations which show cell walls in the position 
shown in the figure. Hence it is not certain that in this species there 
are two lateral derivatives of the generative cell, as seems to be the 
case in the species mentioned by JEFFREY and CHRYSLER (4). 
The dividing nuclei of the species of Podocarpus here investigated 
show uniformly twelve chromosomes in cases where they could be 
certainly counted (figs. 14, 27, 32), and twenty-four were counted 
several times, with less certainty, in the sporogenous tissue of P. sp- 
In this it conforms to the count for all other gymnosperms S0 far 
as known except Taxus, with eight and sixteen (STRASBURGER 12), 
and Sequoia, with sixteen and thirty-two (LAWSON 7). 
It is not possible to speak with certainty concerning the stage at 
which the pollen is shed without knowing over how long a time shed- 
ding continues, and without actually having gathered pollen as it 
is shed naturally. But from cones that were apparently ready to shed 
their pollen, stages were obtained in P. nivalis running from ne 
microspore to gametophytes with two prothallial cells, generative cell, 
and tube nucleus. It often happens that both extremes may be ound 
in the same sporangium. In P. sp. no cones were apparently old 
enough to shed their pollen, though the oldest had essentially the 
same structure as those of the species just mentioned. In the set 
of P. totarra Hallii the range appears to be still wider, for the oldest 
