4° BOTANICAL GAZETTE | JANUA 
A). As the colonies age their color becomes much deeper, 
until in old growths the color is a vivid red. The color is n | 
affected by the ordinary acids or alkalies. The cells show no — 
characteristics of growth under various conditions : 
NUMBER 1. ‘ 
No.| Kind Medium Temp. Color Manner of growth 
10% 
I | Streak | Wort-gelatine | 21° C. Red Abundant, oily in appear- 
z ance, smooth edges 
2 3 7° C. | Pale pink | Slight growth, oily, smooth 
edges 
3 es s ie hae me Red Same as no. I 
4 | Stab m= ar. Red Growth on surface only, 
ys oily, smooth edges 
5 ae 7 ©. | Pale pink | Slight growth, other re- 
spects like no. 4 s 
6 ‘ Agar 21° C.| Deep red | Growth very heavy 
7 Starch paste | 21° C. | Pale pink Slight growth on surface. 
8 Wort at <. i Heavy sediment, liquid tur 
bid, no gas, ring at sur- 
; face : 
9 Bouillon 21° C. | Pale pink | Slight sediment, liquid cl’r, 
. thin granular film 
ie Dextrose 21° C Red Heavy sediment, liquid clr, 
II * ae ° Ns . acy luted 
: 7° C. | Faint pink | Slight growth j 
Glucose at Gy Pink Like no. 10, but less sed 
13 id r . a 7 : 
7° C. | Faint pink | Slight growth : 
on Most abundant growth of 
4 Sucrose Tae & Red sugar solutions, liqui 
slightly turbid, thin fil 
: heavy ring at surface — 
15 Dist. water | 21° C, Very faint | Slight sediment; cell 
16 L . pink mpt 
oie actose | 21° C, Pink Next to 14 in growth, film: 
erm. |Pasteursol.with| 21°C, Pink Sediment in bend of tu 
te ouaet | no gas 
——__| . 
The foregoing table gives a general idea of the condit 
used for the growth of number 1. It was found that the y 
