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1898 | RED YEASTS 4? 
in a stab culture in gelatine or agar gave no growth along the 
needle track, the color being entirely at the surface, this show- 
ing it to be aerobic. Upon Io per cent. wort gelatine and nutrient 
agar the best growths were obtained, and in the light. Various 
temperatures were tested, that between 17°—21° C. being found 
the best. At one time twenty-four varieties of yeast were grown 
at that temperature, and of the twenty-four number I gave the 
most vigorous growth. The color shows sooner and is stronger 
when the growth is made in the light. Inoculations in beer 
wort give a very appreciable growth in two days, the liquid 
becoming turbid, and a sediment forming. No film is formed, 
but in about a week a thin ring appears around the surface of 
the liquid ; this ring increases in size and deepens in color with 
the age of the culture. Fair growths, as indicated by the sediment, _ 
were obtained in bouillon and in distilled water; in the latter, 
however, little color was present. Cultures in sugar solutions — 
sucrose, dextrose, maltose, lactose, and Pasteur solution with 
sugar— were made, and all of them offer good media for growth, 
though varying considerably. Remarkably large growths were 
obtained in lactose and sucrose, these being characterized by 
heavy sediment of a deep red color, heavy surface ring, and 
turbid liquid. A peculiar phase of the growth in sucrose is that 
the cells formed spore-like bodies. In no other of the sugars 
did this occur. Cultures in the sugars and in wort were made 
in fermentation tubes, but no gas was formed, though the cul- 
tures were kept for two months. 
Spore formation was tested in the usual way, vigorously 
growing cells being placed on gypsum blocks. The blocks were 
kept under various conditions of light and temperature, with the 
result that at from 17°-21° C., and in the dark, granulations 
appeared in cells, these afterward forming spore-like bodies. 
The granulations first appeared in about forty hours, though it 
was about four days before the spore-like bodies were formed. 
These bodies are round and highly refractive, averaging 3.3 in 
size, but developed no spore wall that could be distinguished. 
Being doubtful as to the true nature of these bodies, and think- 
