42 BOTANICAL GAZETTE [ JANUA 
ing that probably they might be fat globules, the cells were 
treated with ether, alcohol, osmic acid, potash, and alkanet, all 
of which failed to change the bodies. Some of the cells were 
then placed in a drop of wort in a moist chamber at 25° C. to 
determine their method of germination. The chamber was ~ 
placed under the microscope, so that any changes might be} 
observed. Insixteen hours the bodies had become swollen, and 
they passed gradually from the swollen condition to the ordinary 
condition of the protoplasm in a vegetative cell. The spore 
like bodies formed in the sediment and film of a 5 perce 
sucrose solution were tested in the same way, with like resul 
serving the organism from extinction under adverse conditions. 
Plate III, B, shows the cells from a gypsum block with the 
contained bodies. Growths on blocks kept in the dark and at7 
developed but slight color, while blocks kept at 21° C. de 
oped quite a marked color, both in the dark and in the light, 
thus indicating that a certain amount of heat is necessary ” 
color development. 
NUMBER 2. 
~ Sa aOR emmeerenn ace 
No. Kind Medium | Temp. Color Manner of growth 
I | Streak |Wort-gelatine| 21° C,| Salmon pink | Oily, s a edges, abun- 
dan 
: . Agar . . Oily, aol edges, more 
; abundant than in no. I 
3 Stab |Wort-gelatine)  “ “hy Slight growth along nee- 
4 “ dle trac 
Agar : “ Slight growth along nee- 
dle trac 
5 SS Starch “ “ : 
6 |Ferm.tubel Pasteur sol, i Oily growth on surface 
7 with sugar a Pink Sediment in bed, no gas 
Bouillon Pale pink | Hea mad sediment, liquid 
8 Dextrose és i . « . 3 
n ‘ dclear 
9 Lactose “é ‘“ Heavy sedim Lie ee 
ed Glucose a6 ““ “ ts “ « 
Ir Sucrose bal “s “ 6“ se ‘“ 
— 
inna 
