1898 ] RED YEASTS 43 
The colonies of no. 2 are somewhat indistinct in color until 
they are about ten days old, when they have a yellowish-red 
color. Their general appearance is very nearly the same as those 
of no. I, but when magnified, differences show, as seen in PV. //, 
C. The plate cultures of the three forms, from which the pho- 
tographs were taken, were made at the same time and under 
similar conditions. The photographs were also taken at the 
same time. The cells of no. 2 are round, averaging 4 X 4m in 
size, and bud frequently. In stab cultures a slight growth 
appears along the needle track. In streak cultures, as also in 
colonies and the top of stab cultures, the growth is very oily in 
appearance, and possesses little or no color at first, though this 
develops as the culture ages, until in very old ones the color is 
quite marked. As in no. 1, the color is unaffected by the ordi- 
nary acids and alkalies. In wort and sugar solutions the growth 
is fairly vigorous, though not so much so as inno. 1. No film is 
formed, nor is any gas developed. No spores or spore-like 
bodies are formed either on gypsum blocks or in any of the 
growths, the only change in the cells, when placed under con- 
ditions for spore formation, being a granulation of the proto- 
plasm. 
NUMBER 3. 
No.| Kind | Medium Temp. Color Manner of growth aoe 
1 | Stab Agar |21°C.} Faint pink — Slight growth along needle track, 
surface growth dry, granular, 
abundant 14 
2 | Stab | Gelatine > Pink Like no. I 14 
3 |Streak} Agar # Pink Growth abundant, dry, floury, 
most of color in center in 
heaviest growth, edges almost 
white 20 
4 |Streak| Gelatine | Pink Like no. 3 20 
5 | Stab | Starch A Faint pink | Slight growth on surface 14 
% Wo 4s Pink Turbid after a week, granular 
m, sediment heavy, no gas 20 
7 Lactose a Faint pink | Slight sediment, no film, no gas 14 
8 extrose oe iT7 oe “ ay “ 14 
9 Glucose te ‘ ‘ ‘“ ‘ &s 14 
Io Sucrose “ ‘“ “ “é “ se 14 
