44 BOTANICAL GAZETTE [JANUARY 
In no. 3 the colonies are entirely distinct from those of nos. 
I and 2; they stand out from the gelatine as feathery masses — 
with very irregular edges, and of a salmon pink color. PL/I, — 
D shows the peculiar appearance very well. At first sight the 
colonies look somewhat like a mold, but upon closer inspection 
they show no ramifying filaments. This peculiar appearance of | 
_the colonies is due to the form of the cells, and their remarkable 
method of growth. A few of the cells have the regular round ~ 
or oval shape of the ordinary yeast cell, but the remainder have ‘ 
irregular, swollen, or filamentous forms, resembling the involu- — 
tion forms of bacteria. P/. ///, E is from a vigorous growth on 
gelatine. These forms, with about the same length of filament 
are constant for young growths on solid media, also for the first — 
colonies obtained from exposure to the air. In old growths the — | 
form varies, the filaments becoming much longer, and more — 
branched; this lengthening and branching also takes place in 
cells grown in liquid media. Pl. ///, F is a photograph from am 
old growth. /P/. /1/, G isa photograph from a culture in wort 
The cells, though branching in a manner which resembles 
mold, never develop a mycelium. The cells vary in size from 
1.5@to 3.13 for the short diameter, and 3u to 29m for the lo 
diameter. A fairly vigorous growth is obtained at room tempele — 
ture on agar and wort gelatine. On both of these the growths — 
dry and floury in appearance, of a light pink color, and in stal 
cultures most of the growth is on the surface, only a slight growth 
appearing along the needle track. In wort it takes four or fiv 
and agar. a 
Of the three forms studied not one of them is a true sae 
_charomyces. They resemble the Saccharomycetes in thel 
appearance, both microscopically, and in gross growth, bt 
they are unable to form true ascospores. No. 1 appears t0 
