162 BOTANICAL GAZETTE [MaRcH 
of the cilia of the antherozoids of this cycad, are centrosomes: 
and the same thing appears likely to be the case in Gingko, 
studied earlier by Hirase. 
The following are the results of my recent investigations of — 
the pollen mother cells of various phanerogams (Vymphea alta, 
Nuphar luteum, Limodorum abortivum), examined after fixationin 
Flemming’s fluid and other suitable reagents. 
In Nymphea, which is of particular interest, the adult mother 
cell contains an excentric nucleus, lying very near the wall, the : 
rest of the cell being almost entirely filled with starch, At ~ 
the commencement of the prophases of division the cytoplasm 
assumes a filamentous appearance around the nucleus, in which 
the chromosomes (apparently thirty-two in number) are peripher- 
ally placed in contact with the nuclear membrane, and in addi 
tion to the nucleolus a certain number of very fine achromatic 
threads can be distinguished. 
In the neighborhood of the nucleus certain stains, such’ 
a mixture of methyl-green, fuchsin, and orange G, bring outon — 
or two small differentiated bodies, sometimes of homogento® — 
aspect, sometimes provided with a more deeply stained central : 
granule and sometimes more numerous. At certain stages of 
act as points of attachment for cytoplasmic threads directed ’ 
towards the nucleus, and form the first outline of the nucleit 4 
spindle. Sometimes tripolar or even quadripolar spindles * 
found, but the final figure never has more than two poles, ee ; 
pied by a small sphere which has for its center either 2 5! a 
granule or several granules in a group, whose deep gree? om 4 
brought out by the stain indicated, is like that of the wor : 
Somes. These elements can also be stained by other methods. *" 
account of the small number of multipolar spindles that I a q 
observed, I am not able to suggest, at present, in what ma a 
the multipolar spindles become bipolar. 
The nuclear Spindle forms on the side of the cel’, hich 
curved in accordance with its contour. The two cones ye 
compose it, and whose bases correspond to the nucleate : 
elongate and incurve more and more, until the spindle — : 
