44 BOTANICAL GAZETTE [JANUARY 
nuclear structures black by the haematoxylin; or finally both may 
appear stained red by eosin. Such differences are shown in the 
drawings from the different preparations; the parts shaded in black 
represent portions stained by the haematoxylin, as in jig. 33, those in 
gray the portions stained by the eosin, as in jig. 13. 
Within a quiescent nucleus situated between the two pyrenoids 
thus stained, there can be seen a central body stained somewhat 
redder or blacker, as the case may be, than the peripheral network 
of granules. This network of granules, ordinarily scarcely distin- 
guishable from the cytoplasmic reticulum, was found in some cases 
to be quite conspicuous. 
If an examination is made of a nucleus in process of reconstruc- 
tion from the telophase, within the forming membrane can be seen 
a conglomerate mass of substance, very evidently non-homogeneous 
both in surface view and as seen in outline, figs. 1, 39. Around 
this smaller bodies can be seen in the meshes of a delicate network. 
The staining capacity of the larger mass and the small bodies varies 
in the different preparations; in some instances they are sharply 
defined from one another, at other times they retain the same kind 
and amount of stain. 
It cannot be denied, however, after a careful examination of 
stages preceding the appearance of these bodies, that the substances 
in both came from the chromosomes of the metaphase. Bearing in 
mind, then, that the large mass and the smaller granules have the 
same origin, it would hardly seem correct to discriminate between 
the two, terming the one nucleolus and the others chromatin granules. 
Neither method of staining nor study of their history yields evidence 
other than that they are of similar substance, differing only in position ._ 
and aggregation. It is as if in the revolutions going on within the 
cell some of the chromatin granules had been drawn to the center, 
there incompletely cohering, while others were left at the periphery. 
In describing, then, the quiescent nucleus of Zygnema it seems prefer- 
able to say that the larger portion of the chromatin granules cohere 
to form a central body analogous in its position to the nucleolus of 
higher plants. 
The division of the nucleus is presaged by granules collecting in 
the region where the cell wall will form. The activity of these vibrat- 
