ll teat, 
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SECOND EDITION, 
ILLUSTRATED 
Methods in Plant Histology 
By te J, CHAMBERLAIN, A. nt Ph.D. 
Instructor in Botany in the Unibersity of Chic. 
A Constant Help to Teachers and 
Students of Botany 
Contains Directions for Collecting and Preparing Plant Meterial forMicroscopic 
Inbestigation 
manual to be published on this subject. It is the result of several years’ work 
with classes in residence at the University of Chicago, and with University 
Extension classes away from the University. It aims, therefore, to meet the 
requirements, not only of the student who has the assistance of an instructor in 
a fully equipped laboratory, but also the student who must work by himself and 
with limited apparatus. Free-hand sectioning, the paraffin method, the collodion 
method, and the glycerine method, are treated in considerable detail. In later 
chapters specific directions are given for making such preparations as are needed 
by those who wish to study the plant kingdom from the algae up to the flowering 
plants. Special attention is paid to the staining of karyokinetic figures because 
the student who masters this problem will find little difficulty in differentiating 
other structures. Formulas are given for the reagents commonly used in the 
histological laboratory. 
In preparing the second edition the author has kept in view the advance in the 
science since the book first appeared. Professor Klebs’s methods for securing 
various reproductive phases in the algae and fungi have been outlined in a prac- 
tical way, and in general much more attention has been given to collecting 
material. New chapters deal with the Venetian turpentine method, micro-chemical 
tests, free-hand sections, special methods, and the use of the microscope. These 
changes and additions have enlarged the volume from 168 to 272 pages. 
t is based upon a course in botanical micro-technique, and is the first complete 
272 pp., 8vo, cloth, net, $2.25, postpaid $2.39 
THE UNIVERSITY OF CHICAGO PRESS 
Chicage and New York 
