1906] FULTON—CHEMOTROPISM OF FUNGI 95 
TABLE V 
ne =< 
a Ho < & 
B 5 e a| < F B : E 
CONTRASTED CULTURES IN SILICA JELLY “u& Bog 4B ao #65 
opegen waa: 2 at ER 
Oe |aea Ze ae Ze 
pa 3 on 5 om 
a A a a 
Full nutrient : full nutrient .....2...7........ 35 32 32 33 20 
acking glucose : aesiie pete ce Poe 32 36 37 3° 33 
Lacking glucose : containing glucose........... 27 3° 34 oO 26 
Lacking glucose : full nutrient................ 3 36 33 3° 3° 
Lacking KH,PO, : lacking KH,PO,.......... 92.09 40 Sy On ae Ae 
Lackin aPO, de pein me PO, eran 36 36 31 30 28 
Lacking KH,PO, x fall watrient. toca: ewer 32 a0" 3g 36 | 28 
Lacking (NH,)NO, : lackin ae “CNET Ale Saree as ee a ae Ge we 6 | 28 
Lacking (NH,)NO, : ati aes )NO, eto a 32 32 33 29 
Lacking (NH,)NO, e full nutiaent is. escras ere. 34 33 33 She 3? 
Lacking MgSO, : lackin ng MgSO er ee 33 ar 33 a3 27 
Lacking MgSO, : containing MgsO, setae ade is 39 34 33 33 29 
Lacking MgSO, : full nutrient................ 29 33 32 35 3° 
under conditions that would be presumed to be most favorable for 
chemotropic reaction. 
Tests with epidermis and cabuan jilms.—To test the effect of 
physically different perforated sheets as well as effectually to repeat 
the methods used by former investigators, use was made of celloidin 
films which had been perforated, and of strips of epidermis of Yucca 
aloifolia. This gave, with reference to physical properties, a range 
from the wholly impermeable mica plates on the one hand to the 
semipermeable celloidin films on the other. 
The tests with epidermis were made with Monilia fructigena, 
Sierigmatocystis nigra, Botrytis vulgaris, Sphaeropsis malorum, and 
Mucor stolonijer, the spores of which were distributed in non-nutrient 
8 per cent. gelatin above the epidermis in its final position; gelatin 
layers containing 5 per cent. cane sugar, 4.5 per cent. dextrose, 
0.01 per cent. copper sulfate, 0.1 per cent. oxalic acid, 0.2 per cent. 
phosphoric acid, and non-nutrient gelatin, were spread below the 
epidermis. Under these conditions the penetration of the stomates 
or turning toward the stomates was practically zero. When no 
culture medium was used, the spores being merely spread on the 
under surface of the epidermis, hyphal growéh was good. A few 
hyphae of each species grew through the stomates; but there was 
no evident turning toward them, and in no case was there pene- 
tration of more than one or two per cent. of the stomates. 
