386 BOTANICAL GAZETTE [JUNE 
1. Tissue packed full of ice in shoot and in mesophyll of scales forming 
sheets parallel with the surface; rapidly and completely reabsorbed when the 
sections were thawed in oil. Sponging out of sections very marked.—Populus 
dilatata (fig. 2) and P. candicans, Prunus serotina and P. virginiana, Betula 
enta, Acer Negundo, Pyrus Malus and P. communis, Aesculus Hippocasta- 
num. 
2. Containing a large amount of ice, but the water tardily reabsorbed on 
thawing in oil.—Acer Saccharym, Tilia americana, Ulmus scabra, Crataegus 
punctata (jig. 3). 
Fic. 3.—Crataegus punctata: cross-section of bud, showing ice in bud-scales and 
floral parts. 
3. No ice could be found at o°C. Tissue dense, of small cells.—Castanea 
dentata, Hamamelis virginiana, Fagus americana, Fraxinus americana, Jug- 
lans cinerea, Corylus rostrata, Quercus alba, Hicoria ovata. 
4. Other cases—In Pinus Strobus and P. sylvestris there was a moderate 
amount of ice in the shoot and in the anther as well as in the inner scales. In 
Syringa vulgaris there was a very large quantity of ice in the scales and young 
shoot, especially in the anthers (fig. 4). In Viburnum dentatum and Prunus 
persica the amount of ice was small, but water was quickly reabsorbed. 
