416 BOTANICAL GAZETTE (JUNE 
The buds in the above experiment were separated from the 
tree upon which they grew, and therefore could not receive water 
from it to replace that transpired. It would be interesting to know 
how much water moves into the bud to replace the quantity lost, 
thus giving a better idea of the actual decrease in percentage within 
the cells. This has not been done for twigs at temperatures above 
freezing, but the following figures are available for the frozen buds 
of pine. I selected six vigorous buds of Pinus Laricio, all on the 
west side of the tree, deprived them of their bud-scales and allowed 
them to remain exposed three days. The temperature during 
this time ranged from —18° C. to —6.7°C., so that the twigs as well 
as the buds were constantly frozen. Three of the buds were cut 
off, the cut surface sealed, and placed in a tray at the base of the 
tree, while the other three remained attached. The results were 
as follows: 
baie’ pala Dry weight | Difference | Per cent. ™ wet at end 
Cut buds— 
i ©.1058™| 0.0618™| 0.044 41.9 | 
" 2c Sk aati eg aR PE Tae PP 0.150 0.090 0.060 pies Maas average 
Ree eed ree os 0.230 0.134 0.096 41.8 
Sra Bas coe 
No Ae Sy eo 255 65139 0.116 45-5 
an 0.240 0.121 0.119 49-5 747-5 average 
Des aie cP ees a es 0.210 0.110 0.100 47.6 
It seems, therefore, that there was a rise of about 5% of water 
into the bud while the tissues were frozen. This is quite possible, 
since only a portion of the water was converted into ice, the remainder 
remaining fluid in the walls and protoplasm and still capable of 
movement. The figures given above for the loss of water from 
desquamated buds are therefore slightly too large in every case. 
It may be noticed by computing corresponding figures that the loss 
of water during this last experiment is slightly less than in the two 
previous experiments in which desquamated buds of Pinus Laricio 
were used. This was due to the fact that the last experiment was 
conducted in a different place, on the other side of the building. 
‘I have found that exposure makes a very considerable difference 
in the loss of water, and readings which are to be compared must 
