1899] STUDIES ON REDUCTION IN PLANTS 17 
transversely at the convex end and the two rods lie side by side 
or near each other. The nuclear membrane now disappears and 
the kinoplasmic threads enter to form the spindle for the second 
division. 
The elongation of the nucleus while the chromosomes are 
moving to the poles shows that the forces are in play which 
form the spindle for the second division. It is evident also 
that the chromosomes remain distinct, and that they are soon to 
be again separated transversely. 
The elongated form of the nucleus marks the position of 
the spindle for the second division, and the poles of the spindle 
lie near the poles of the elongated nucleus. The spindle is 
therefore inequilateral, the poles being curved toward the cell 
plate of the first spindle figure. At the same time the chromo- 
somes begin to move inward to the cell plate, and in doing so 
show the same general form which they possessed during the 
anaphase of the heterotypic division, which it seems they possess 
through the short period which intervenes before the formation 
of the second spindle figure. 
During this time there is no longitudinal cleavage of the 
chromosomes. Even should the chromosomes form a continu- 
ous band in the daughter nucleus of the first division, there is no 
longitudinal splitting of the same at this time. Sometimes there 
is an appearance of a longitudinal fission of the chromosomes 
where the edges seem to be more deeply stained than a middle 
zone along the axis. This was found to be due, however, to the 
deeper staining of an outer layer of the large chromosome, and in 
properly stained preparations can be seen at any stage of mitosis. 
If longitudinal fission of the chromosomes takes place during 
the second division, it would then be sought for in the nuclear 
plate stage. 
Since the chromosomes during the prophase of the second 
mitosis are of the same general form as those of the anaphase of 
the first division, and very likely preserve their identity through 
the short intervening period, they should be chiefly V- or U- 
Shaped. This is the case, as the examination of a large number 
