: 
; 
| 
1899] MICROSPORANGIA OF HEMEROCALLIS FULVA 83 
they must be drawn entirely within the dark layer before the 
nuclear membrane has disappeared. 
A careful study was made of preparations containing cells in 
various stages of division, many of which showed distinctly 
dome-shaped bipolar spindles just after the nuclear membrane 
had disappeared. Occasionally a nucleus was observed which 
seemed to have radiations extending outward, but these were 
never sufficiently distinct to be considered as forming a multi- 
polar spindle. The spindle was not differentiated by the stains 
in any case until after the nuclear membrane had almost 
disappeared, as the dark band obscured the poles. At first 
these alone would be visible, since the remainder of the spindle 
could scarcely be distinguished from the nuclear membrane 
because of their close proximity; the central part of the spindle 
perhaps being in contact with the nuclear membrane. The 
spindle fibers at this stage are delicate, the spindle being in 
Process of formation. The fact that the nuclear membrane 
disappears while the spindle is forming lends support to the 
theory that the material of the nuclear membrane is consumed 
In spindle formation. 
As the poles separate, the spindle gradually becomes pointed 
and seems to grow somewhat narrower in the middle portion, so 
that it is not as wide as was the original nucleus ( figs. I-7, 
10-12). ‘The chromosomes are drawn into the equatorial plane 
(figs. 8,74) soon after the spindle becomes elongated to definite 
ia Small deep-staining bodies which have the appearance 
ye gaa are often found at the poles in the various stages 
. — yaaa divisions (Jigs. 9: 9 i Ea) ‘ 
idler ce 3: i vision. of the spore-mother-cell the bi 
Re as oe successively (fig. 22), but almost always 
Ge ies Y (figs. 34, 76), the spindles usually being parallel 
16), and oa (Jig. 15), but occasionally obliquely placed (fig. 
€n persisting even after division is complete (fg: 
17). 
ah e - Phenomena of the first and second divisions are the 
band j Xcept that in the latter the nuclei are smaller and the dark 
1s i 
not so conspicuous, 
