90 BOTANICAL GAZETTE [avons 
from specimens that had been fixed for twenty-four hours ina 
weak Flemming solution of the following formula: 1 per cet 
chromic acid 25°, 1 per cent. glacial acetic acid 10%, 1 per ceil 
osmic acid 10°, water 55°°. Much material, however, was killed 
killing agents. The results of these experiments are brougit 
together at the end of the paper and present some interestilf 
data. 
Sections were cut 5 and 7.5 ~ thick from material embeddel 
ing to the principles of Haidenhain. Acid fuchsin followed bj 
methyl green or gentian violet presented interesting examples# 
differential staining, but the results were not as good as tho 
given by Flemming’s triple stain. 
At the outset it will be well to state that the number® 
chromosomes for A. /aevis appears to be four and eight in ie 
_ 8ametophyte and sporophyte respectively. The count in me 
gametophyte is based solely upon observations cf many ie. 
figures in the cells of a developing antheridium, but it mee 
with the number presented in the nuclear figures i _ 
spore-mother-cell. The number eight for the sporophytic g® | 
eration was presented quite frequently and in different regio® : 
of that structure. Thus the archesporium just before the diffet 
entiation of the spore-mother-cells, the tissue below the at 
$3 
it 
time when the spore-mother-cell is differentiated until the spore 
are fully ripe. It is possible from this fact to relate te 
other with great exactness the events that take place 8 © 
Spore-mother-cell matures. 
