154 BOTANICAL GAZETTE | SEPTEMBER 
counted twice in adjacent sections, it would perhaps be fairto 
place the average at about 250. Wager found 115 in A. candidu, 
and Berlese 200 in A. Portulace. It is very difficult to makean _ 
accurate count in the antheridium, because this structure isso — 
small and of such irregular shape that it is impossible to recog: 
nize its limits in adjacent sections. However, an average based 
on several counts indicates that the number is likely to be about 
35. This number is considerably greater than that suggested 
by either Wager or Berlese, both of whom report about 12 mud 
in the antheridium. : 
When the oogonial nuclei are passing into the spirem cont! 
tion the Hautschicht seems to be closely appressed to the wall 
the vicinity of the antheridium. This fact is demonstrated mo 
clearly in preparations where there has been slight collapse 0! 
the contents of the oogonium ( figs. 47, 48) and the protoplasm has ; 
shrunken away from the wall everywhere excepting at the pois | 
opposite the antheridium. This adherence of the Hautschicht 
correlated with a very marked granulation of the cytoplasm # 
this region, a phenomenon also noted by Wager, and one whit 
seems to be significant. It suggests that a cellulose enn 
is secreted to dissolve the wall of the oogonium. As | i 
cated in figs. 48, 49, 50, this wall frequently shows the marks © : 
corrosion over a considerable area, always at a point oppos | 
the antheridium. This interesting process results in 4 neat ‘ ; 
foration, through which the cytoplasm of the oogonium flows a 
as to form a very conspicuous swollen papilla within the ” 4 
idium. Various stages in this process are shown in jigs — 
It is difficult to explain this phenomenon. The initiatory — 
the perforation of the oogonium wall seems without ag 
taken by the protoplasm of the oogonium itself. But oy 
the significance of the pushing of the cytoplasm of the ee 7 
into the antheridium to form the peculiar bubble-like papi 2 
The structure, both wall and contents, stains deeply, thus a 
ing very conspicuous, while its extremely frequent -« ue 
as well as its presence in other species, seems to in eer 
is not abnormal. The papilla wall is so extremely thin : 
