1899] THE COMPOUND OOSPHERE OF ALBUGO BLITI 159 
and the other to the periplasm, and the line of differentiation is 
sharply defined and unmistakable. Nuclei may be observed in 
every phase of this mitosis, and the daughter nuclei may be 
found in all stages of reorganization, one of each pair in the 
ooplasm the other in the periplasm. The writer was unable to 
detect any difference between the mitoses that occur strictly in 
the periplasm and those that contribute daughter nuclei to the 
oosphere. Asa result of the division a large number of nuclei 
pass into the ooplasm, thus producing a multinucleate cell con- 
taining by actual count an average of 45 to 55 nuclei (not 
- than 40 or more than 60). The oosphere is thus a coenocyte; 
instead of the uninucleate cell which one would expect there is 
found a multinucleate structure, to designate which the writer 
has used the term compound oosphere. 
Because of the importance of the anomalous compound 
Sosphere and the peculiarities of its development, it seems best 
to discuss at length difficulties that might be suggested. It may 
be claimed that amistake has been made in the sequence of 
Sree, and that the multinucleate condition of the central 
“Bion does not follow, but precedes zonation. This objection 
aoa for four reasons: (1) the sequence is complete up to 
saa there is no place for a multinucleate central region 
white va ? the studies in cytology make it certain ee sa 
Phase of the Sealine ie i is ponies eae 
thus renderin as an in igs of the age o 8 ‘ 
ble: £3). § any misunderstanding of the sequence imposs 
standing of ig ctation Picea is necessary to the “— 
(4) the _. that are positively and unmistakably older; 
opment of the antheridial tube and central body 
with the views presented. It would seem impossible 
Stake in seriation has been made. It may be claimed 
™ sity, os nuclei seen in the mature oosphere are not 
idence to . Salt = by the knife. There is abundant 
- «Often die in ophaibds this claim, for the nuclei in west 
Must have be : is no that, if carried in by the knife, they 
©n carried in two opposite directions by the same 
ta mi 
“lat the 
