160 BOTANICAL GAZETTE [SEPTEMBER 
stroke. It may be well to state that specimens of every structure 
or stage represented or described in this article are preserved, 
not in one, but in several mounts, and for most of the important 
stages in many preparations. No isolated or single fact is any- 
where used either to support or destroy any theory. 
With the entrance of the nuclei differentiation of the oosphere 
is complete. The ooplasm is of very fine, even texture, made 
up of such small meshes that the vacuoles are never more that 
half the size of the nuclei, and there are no prominent granules 
or oil drops shown by the Flemming stains at this time (figs.6e 
69). The periplasm is loosely vacuolate, the strands are often 
granular, and the nuclei are frequently at their intersection, — 
often in bunches. : 
The nuclei of the antheridium usually divide simultaneously 
with those of the oogonium, this being so constantly true see 
from a glimpse of one organ the condition of the other could 
be predicted. No difference between mitosis in the oogontum 
and in the antheridium could be observed. At the time of 
differentiation of the oosphere the contents of the anther! 
tube stain deeply, but it has not been possible to demonstrate 
nuclei in its interior at this age. When the nuclei line up P* 
paratory to zonation the antheridial tube has usually peneue® 
the periplasm almost to the outer boundary of the oosphe ; 
and as it later pushes into the oosphere, during the ae 
of the mitosis, it somewhat indents the boundary film. © 
oogonium was seen that had two antheridial tubes pe ge 
the periplasm from opposite sides, but neither had yet Pl 
the boundary of the oosphere. Another stage Wa " 
where an unemptied tube lay tangential to the 005 
apparently the unfavored of two competitors. Still 
presented a stage of fertilization in which two tubes wee 
taneously opening into one oosphere. 
Minute globules of brownish color are to 
cytoplasm of the oogonium from its earliest devel 
zonation. In all early stages they are indefinite ! 
irregular in size, but seldom larger than one © 
! 
be seen it 
lopment up 
n num . 
f the ® 
