164 BOTANICAL GAZETTE [SEPTEMBER 
are particularly noticeable in the fine meshed area of the 
oosphere. They do not appear when stained in the ordinary 
way by Flemming’s triple stain, but seem always present and com 
spicuous if stained by Heidenhain’s hematoxylin, when they 
are black ( fig. 75). A hematoxylin preparation when bleacheé 
and restained for a long time in safranin shows numerous rou 
red bodies in the same position. In appearance they are a trifle 
longer than broad and often double, as though two were lying 
end to end, reminding one of large bacilli. It does not seem 
probable that these coalesce to form the central dot, as might 
be suggested by fig. 75, since they have a different reaction t0 
stain. 
SIMULTANEOUS MITOSES IN THE OOGONIUM. 
During the differentiation of the oosphere the nuclei in the 
oogonium divide once ( figs. 58-62, 65-67), the mitosis occurilg | 
about simultaneously for all of the nuclei, cases of independes 
division of single nuclei never being found. Almost invariably # 
oogonium in the condition of zonation presents the nucle! # 
metaphase, or just passing into anaphase ( fig. 65). In ee 
stages, just before zonation, when the cytoplasm is massed we 
one or several centers, the nuclei are usually in prophase Ve 
59-62), but metaphase may be present even as early * i. 
beginning of this process (fig. 58). It is apparent that anaes 
is never reached until after zonation, and that the mitoses eA 
when the cytoplasm commences to collect in mae 
nuclear division always occurs when the antheridial “ te 
the position shown in fig. 59. It would seem, ede sat 
differentiation of the oosphere would take place rapidly 1 . 
consists merely in a floating out of the vacuoles ant Sa 
the interior region of the oogonium, but mitosis 15 Pe 4 
less rapid. If changed conditions should hasten 0! ss 8 
process of zonation, one would expect a variation oe sah 
exist in the time correlations between zonation and the 
The spirem condition of the nucleus has been ma 
the other stages of nuclear division have not been 
