166 BOTANICAL GAZETTE [ SEPTEMBER 
differentiated. The dark strand shown in the lower half of fp — 
8 is probably like the less conspicuous one in a similar position 
in fig. 5. Both may be considered as the remains of a spitem 
thread, such as is shown in fig. g. ig. 7 is open to a similat 
explanation. The difference in the shape of these two figuresis 
noteworthy, since it is probably due to their position in the 
oogonium. The spindle shown in fig. 8 lay in a strand of pet | 
plasm which supported an oosphere, similar to the strands shows 
in fig. 62. It is probable that the length of the spindle is due 
to the tension to which it was subjected. Fig. 7 was from’ 
crowded bunch of nuclei, and could not elongate. The apps 
ance shown in this figure might tend to support the idea that the 
spindle fibers are formed from the linin thread, a view enles 
tained by Wager, but disputed by Berlese. The question p© 
sents so many difficulties that the writer does not feel warrante! 
in expressing an opinion. 
The nucleolus at the time of late propha eee 
small, but often quite as large as when the nucleus 1s | 
spirem condition. It may be found throughout all stages of 
mitoses. ig. 12 shows the splitting of the chromo ; 
jig. 13 may be recognized as a condition immediately ee | 
membrane is still intact and encloses the nucleolus which — 
outside of the spindle, and the centrosomes are at their maxinil® | 
definiteness. It is interesting to note in passing a 
nuclei lying very near to the antheridial tube are usually on 
a full phase in advance of other oogonial nuclei in ess 
ar * 
fact strikingly apparent when the majority of the nuclel c 
metaphase. 
The chromosomes, after the division of th 
move poleward with unequal rapidity, the poles 
character, and the nuclear membrane is no 10 
boundary of the nucleus being marked by the Sf pul 
(fig. 14). With the loss of the membrane the wed 5 
structure assumes, and retains through later stage pe 5 | 
of staining more darkly, a character particularly aah 
the regions where the chromosomes lie ( figs. aS ee . 
se is sometime 
spindle a 
