1899] THE COMPOUND OOSPHERE OF ALBUGO BLITI 169 
esting to note that the two nuclear divisions in the antheridium 
and in the oogonium are similar in character and proceed simul- 
taneously. The antheridial tube at the time of the differentia- 
tion of the oosphere lies in the periplasm, with its apex close to 
the bounding film of the oosphere. It now pushes into the 
oosphere, increasing in diameter as it advances. Ititakes safranin 
stain greedily from this time until it discharges its contents, but. 
if the stain be thoroughly extracted in acid alcohol and the 
preparation treated with gentian violet the contents become 
clear. The tube when fully developed is seen to contain numer- 
ous nuclei. A glance at figs. 73, 76 will give a clear notion of 
this condition. It will be seen that many nuclei are massed 
near the tip of the tube and that others are apparently entering 
at the base. It is impossible to determine their number by 
actual count, Owing to the crowded condition (jigs. 77, 85, etc.). 
However, as there are about 35 nuclei originally present in an 
antheridium, and these divide twice, there must be altogether 
ent 140. Of these, 20 or 30 perhaps remain in the anther- 
idium Proper, leaving a little more than 100 to pass into the tube. 
The antheridial tube pushes toward the center of the oosphere 
during the Second mitosis (fig. 70), and arriving nearly at the 
“enter its tip swells, becoming nearly globular. In this condi- 
tion the end of the tube is covered by a very thin wall which is 
tely visible, and yet holds within a dense mass of sperm nuclei 
S77). 
When the male n 
the ch 
anterior ; » and later pointed at both ends, and the 
énd is seen to contain the nucleolus around which is 
 * Massed a densely staining substance, probably chromatin. igs. 
rtd aly nuclei from both the base and tip of the same tube, 
Which is illustrated in Jig. 73. In the narrow entrance and 
*! Portion of the tube the nuclei are necessarily arranged in 
a ile, but as its diameter enlarges they become massed in 
ei and the tip is so closely packed with nuclei that 
| one forcibly of the appearance of a raspberry with its 
Sin 
