170 BOTANICAL GAZETTE [ SEPTEMBER 
drupelets (fig. 77). Two sections of the same antheridial tube 
are shown in figs. 77, 78, one at the tip showing numerous nucle 
surrounded by a very delicate membrane, the other near the 
base giving a view of the narrow nearly empty cavity and the 
thick wall. As the tube enlarges the protoplasm in the anther 
idium proper becomes more and more vacuolate, but its conten’ 
never entirely leave the structure (figs. Soa, 86). The film 
separating ooplasm from periplasm is but slightly if at all 
changed by the entrance of the antheridial tube and during the 
maturation of the oosphere. The periplasm likewise shows 10 — 
important changes. Some of its nuclei divide mitotically, # 
the number does not seem to increase materially. Most of them 
remain in a resting condition. One case was observed wher 
every nucleus in the periplasm was undergoing mitotic division 
simultaneously with those of the oosphere, but this must be 
regarded as a very excéptional instance. 
FERTILIZATION. 
100 in number, are in resting condition. The antheri 
filled by an approximately equal number of male nuclei, 4 . 
tip has swollen so that the contents are separated fot : 
ooplasm by only the thinnest of walls. The wall finally 
ishes and the contents of the tube are free to mingle ee 
cytoplasm of the oosphere (figs. 80, 82). The pore 
move through the ooplasm toward the female nuclei, oe 
being often marked by a streak of denser cytoplasm. ak 
no visible cause of this movement, but as the male and ! i 
pronuclei differ in form a chemotropic influence may perhaps 
safely inferred. Longitudinal sections of antheridial tubes 7 
80, 82) sometimes show the nuclei pouring out, and pee =. 
or oblique sections (figs. 84, 85, 86, 77, 79) a thee 
proof of a discharge of many nuclei. There is in ange 
sections unmistakable evidence of a multinucleate disch of 
Sections 
the antheridium into a multinucleate oosphere. 
