ies 
* 
Of melted 
1899] BACTERIAL DISEASE OF THE SUGAR BEET 181 
to the healthy beets. The heart leaves of the diseased beets 
were more easily injured by the frost. It is characteristic of the 
disease that the leaves of badly diseased roots die away until no 
green leaves remain, leaving an apparently dead root in the soil, 
though its tissues will be found to be firm and not in the least 
broken down. The early frosts hasten the destruction of the 
leaves. Both diseased and healthy roots show an acid reaction, 
the diseased seeming slightly more acid than the healthy. 
ETIOLOGICAT HISTOLOGY. 
Comparative study was made of sections taken from both 
diseased beet roots and healthy beet roots, also sections from 
leaf and leaf-stalk of both diseased and healthy beets. In all 
these sections small round bodies were seen in the cell sub- 
stance. These bodies were found to be protein by their reac- 
tion to iodine. They measured from 2-4m in diameter and 
turned yellow when treated with iodine. In the tissues of 
diseased beets other bodies were found which were smaller and 
of a different refractive power and arrangement. These bodies 
‘tained in gentian violet like bacteria, and looked almost like 
“rococci when imbedded in the cell substance, but when free 
nthe water were easily distinguished as small motile bacilli. 
SEPARATION OF THE SPECIFIC GERM. 
he first Steps im the Separation of the germ were as follows. 
T 
“seased beet was selected, a thin knife sterilized in the flame 
<a “move all parts of the beet exposed to the air. A 
epee of beet was then removed from the heart of the root 
ey “ansferred by means of a sterilized platinum wire to tubes 
8elatin or agar. The first series of cultures was made 
ting Selatin tubes with pieces of diseased tissue, at the 
a inoculating a number of tubes in the same manner with 
a beet ‘as a control. The following tables ite 
Tesults i of a Series of such inoculations. The first shows ed 
Tsults of Cultures made from diseased tissue; the second 
° cultures made from healthy are 
