182 BOTANICAL GAZETTE [SEPTEMBER 
TABLE I. 
CULTURES ON ARTIFICIAL MEDIA FROM DISEASED AND 
HEALTHY BEETS. 
Number 
Dates and media of Results 
cultures : 
| eee : 
FROM DISEASED BEETS. : 
ee 
Sept. 20 10 Oct, Oct. 10 
3 Gelatin - - Chivaccuteae growth Contaminated 
t. 2 3 eta ee 
Gelatin - - Characteristic growth Still pure 
Oct. 2 4 Oct. 2 
Glycerin gelatin Characteristic growth 
Oct. 24 4 Oct.:2 
Glucose gelatin Characteristic growth 
| init 
FROM HEALTHY BEETS. E 
Sept. 20 Oct. Oct. 10 a 
O Gelatin - - 4 - growth : . No grow? : 
chs, 2 Oct. 45 e ; 
Gelatin - - 3 No growth . No go : 
Oct. 24 Oct. 28 j 
Glycerin gelatin 4 No growth ] 
Oct." a 
Glucose gelatin ) 4 
| 4 
All the above cultures were made in standard gelatin not it ) 
ted or in standard agar to which had been added 5 pee 
glycerin or 5 per cent. glucose. The growth in successitl 7 
ulations was the same in all cases. Some creamy white ee 
grew out on either side of the diseased tissue, and in the 
of a few days were surrounded by a lens-shape¢ °F | 
in the gelatin caused by the gas that was given off int cbt 
When the bubbles reached the surface the growth w 
in white rings around the tube (p/ade XX, By eel 
October 15 several Aries were made in sands 
In some of these a growth resulted but no successtt ea 
were made. One of these cultures was used to in . 
beet in the field. 
5 Photograph of tubes at this stage shown in plate XX. 
