1899] BACTERIAL DISEASE OF THE SUGAR BEET 183 
November 3 stab cultures were made from a tube of glycerin 
agar inoculated with diseased tissue. These were all contami- 
nated with the exception of one which formed a perfectly color- 
less layer, gelatinous in consistence, on agar and sterilized beet. . 
This form will be spoken of later. 
No growth resulted from a series of cultures made in 10 per 
cent. cane sugar gelatin. 
In December a diseased beet, which was frozen the previous 
aight, was brought in from the field. Pieces of this tissue were 
transferred with the usual precautions to tubes of melted gelatin 
‘o which had been added 5 per cent. of cane sugar. The growth 
in these cultures was rapid and gas was given off in large quan- 
ties, Stab cultures were made from these and appeared exactly 
uniform, and just the same in appearance as stab agar cultures 
made directly from unfrozen diseased beet, with one exception 
meach case. From one of these exceptional tubes the perfectly 
colorless gelatinous form spoken of above was found. This form 
"as also obtained in one of the stabs taken directly from the 
diseased beet, 
lag above inoculations the growth was much more rapid 
Previous cultures, probably because the tissues were 
broken down by freezing so that the germ could escape more 
“asily into the ; : 
Surrounding medium. 
: Searal Series of cultures was made at the same time om 
¢t in which the disease was produced by inoculation. 
fers . wth was similar to that of the preceding series. Trans- 
above *n from these were uniform and similar to those described 
ae inoculations a plug was removed from the healthy 
e plug aa @ sterilized knife, the inoculating material inserted, 
Page nile aced and covered with cotton. The table on the next 
The Sey results of inoculations of three beets in the field. 
ing the a oculated with diseased tissue was slower in show- 
ON gelatin “Se than the one inoculated with the germ growing 
ake TY because of the time required for the germ 
way through the cell walls of the tissue. 
