1899] BACTERIAL DISEASE OF THE SUGAR BEET 185 
seems to revolve more or less irregularly on its axis. The germ 
stained well with all the common bacterial stains. No process 
of staining showed the presence of spores or flagella ( plate 
EFFECT OF LIGHT ON GROWTH. 
The germ grew better in the dark than in the light. Germs 
taken from old, dried out cultures were smaller than when grown 
on a moist substratum. Desiccation also injures the capability 
of the germ for motion. Germs taken from an old culture and 
examined in a drop of water were less motile than those taken 
from a fresh bouillon culture. 
The germ grows better at a temperature of 12°-14° than at 
21°. Stab cultures in agar grew slowly at body temperature. 
The germ grown in bouillon and exposed to a temperature of 
100° for five minutes was killed. 
GROWTH OF THE GERM ON DIFFERENT CULTURE MEDIA. 
Stab cultures of gelatin showed a thin grayish-white layer 
baad surface, and extending down the line of inoculation. As 
the cultures Stew older the color darkened to a deep cream. 
The gelatin was liquefied in the course of several weeks. When 
Sige gelatin was inoculated and then allowed to solidify there 
nai growth throughout the gelatin in streaks and films. 
‘SS on gelatin plates were not distinctly outlined and were 
seonnmes accompanied by a disagreeable odor. The germ 
Soa Stow better on agar than gelatin. Agar to which had 
eed 3 per cent. of cane sugar or glucose seemed to 
Paps lavor its growth. The growth on slant agar was drab- 
growth og Smooth margins, and a slow and not luxuriant 
ta 6 © growth was not viscous. 
layers Sar plates the colonies have their origin in the deeper 
of the agar where they are generally elliptical. When 
ot f : ; i i d grayish- 
White Coloni urface they spread out in their round gr y 
..» With compact creamy-white centers. In bouillon 
fluid “eg observed after two or three days. No turbidity of the 
observed, but a sediment was deposited in the bottom 
