186 BOTANICAL GAZETTE [ SEPTEMBER 
of the tube. Masses of zoogloea were sometimes found in 
old bouillon cultures. The germ grew well on sterilized sugar 
beet, and deposited a sediment in sterilized beet juice. The 
_ germ grew on sterilized apple, potato, and turnip. A rawpotato 
was broken open and inoculated with the germ, There wasa 
slight growth developed. A raw sugar beet was inoculated 
The germ grew, causing a black coloration of the fibrovasculat 
bundles, and in a microscopical examination was seen to have 
entered the tissue. | 
NITRATE SOLUTION, 
This solution was prepared using 1000 distilled water, | 
gram peptone, and 1 gram potassium nitrate. Tubes of this 
solution in which the germ had grown for three days when tested 
showed that the nitrate had been fairly well reduced. 
ACID AND ALKALINE MEDIA. 
It has been stated that the beet root is acid to the extent of 
little over 1 per cent. Because of this fact experiments were made | 
with acid and alkaline media in order to determine which e 
two would be more favorable to the growth of the gam : 
bouillon, to which had been added 1 per cent. malic acid, thesoluti® 
was made neutral; 5 per cent. acid solutions were not rend a. 
neutral. The solution was not made turbid by the growth wee : 
germ. In 1 percent. alkaline solution of bouillon the gem a 
more motile than in 1 per cent. acid solution. In iv : 
alkaline solutions the germ was more motile and larger, than | 
grown in acid media, measuring from I.1I-1.9 p long; and 09 . 
broad. In 5 per cent. alkaline media the sediment ae : 
was quite viscous. Zoogloea masses were found more” or 
abundantly in all the alkaline cultures. These solulio® 
not made acid in reaction by the growth of the germ. 
STARCH SOLUTIONS. 
f starch fits 
In a solution composed of one part each oO 
and bouillon, the germ grew but the starch was 
These cultures were tested for starch and gave 
not re 
a gecide® 
