1899] BACTERIAL DISEASE OF THE SUGAR BEET 187 
reaction; they were also tested with Fehling’s solution for 
glucose, but gave no reaction. The germ grew well in wort 
gelatin.” When this gelatin was melted, and after being inocu- 
lated was allowed to solidify, the germ grew under the surface, 
producing bubbles of gas all through the gelatin. 
Tests made of bouillon containing cane sugar in which the 
germ had grown gave a reaction for glucose. In order to deter- 
mine if the enzyme existed outside the cell, a solution in which 
the germ had grown was filtered through a porous cup. This 
filtrate added to a 5 per cent. cane sugar solution, and tested 
with Fehling’s solution for glucose gave no reaction. Further 
*xperiments are necessary before deciding definitely in regard 
to the enzyme properties of the germ. 
CELLULOSE SOLUTIONS. 
As the germ penetrates the cell wall of the plant in some 
"ay, €xperiments were made to determine its effect on cel- 
— For this a special nutrient solution was prepared as 
Ollows :7 
ee a ee 
a : : - - 2.5 gram 
Magnesium sulfate, ‘ : : os 
Calcium phosphate, - : S é . 2 ac f 
Ammonium sulfate, a er 
Sodium chlorid, ‘ s : ‘ ‘ s 1.25“ 
Beef extract, . ; % : : és aes 
Swedish filter. 
* ® this solution the growth was very slow, and a very small 
ting of gas was produced, and the cellulose slightly broken 
FERMENTATION.® 
of the abundance of gas produced by the germ in 
Special fermentation solutions were prepared. The 
“Wort 8elatin was 
iy, ™ la fermentati 
: Because 
"S growth, 
made by adding 10 per cent. of gelatin to wort. 
on de la cellulose. Centralblatt fiir Parasitenkunde 2: 358. 
* Paste } 
0 fermen re H.and EMMa: A ‘report concerning gases produced by bacteria 
Centralb. f, Parasitenkunde 2:707. Dec. 1896. 
